MERS Coronavirus Methods and Protocols PDF

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PART I: Evolution and Entry of MERS-Coronavirus
1. Studying Evolutionary Adaptation of MERS-CoV
2. Evaluating MERS-CoV Entry Pathways
3. Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing
4. Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein
PART II: Genetic Alteration and Structural Determination of MERS-Coronavirus Proteins
5. Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV
6. Deducing the Crystal Structure of MERS-CoV Helicase

PART III: Quantitation of Virus and Anti-Viral Factors
7. Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans
8. Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR
9. Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay Abdullah Algaissi and Anwar M Hashem
10. Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera Abdulrahman Almasaud, Naif Khalaf Alharbi, and Anwar M Hashem
11. Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA

PART IV: Mouse Models for MERS -Coronavirus
12. Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced
13. Development of a Mouse Adapted MERS Coronavirus
14. Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection
15. Evaluation of Activation and Inflammatory Activity of Myeloid Cells During
16. Histopathologic Evaluation and Scoring of Viral Lung Infection David K. Meyerholz and Amanda P. Beck

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